Activision games published an Spider-Man 2 video game on June 28, 2004 to tie in with the release of the movie. The game was developed by Treyarch.
In this third-person action game, Spider-Man now battles Doctor Octopus.
- Nintendo Game Boy Advance
- Nintendo Game Cube
- Nintendo DS
- Playstation 2
- Sony PSP
- Tobey Maguire: Peter Parker/Spider-Man
- Kirsten Dunst: Mary Jane Watson
- Alfred Molina: Otto Octavius/Doctor Octopus
- Holly Fields: Black Cat
- John DiMaggio: Rhino/Alex O'Hirn
- Michael Beattie: Shocker/Herman Schultz
- James Arnold Taylor: Mysterio/Quentin Beck
- Angela V. Shelton: Calypso
- Josh Keaton: Harry Osborn
- Jay Gordon and J.K. Simmons (in the PSP console): J. Jonah Jameson
- Mindy Sterling: May Parker
- Bethany Rhoades: Betty Brant
- Jeff Coopwood: Robbie Robertson
- Joe Alaskey: Curt Connors
- Dee Bradley Baker: Puma
Also features the voice of Bruce Campbell as the tour guide, and the hint markers.
- Quentin Beck/Mysterio‡
- Herman Shultz/Shocker‡
- Alex O'Hirn/Rhino‡
- Otto Octavius/Doctor Octopus‡
‡ The character is a Boss.