Blade: Trinity was released on DVD on April 26, 2005 with both The "R" Rated and Unrated verison and on Blu Ray on July 7, 2012 with the Unrated only.
- Widescreen version of the unrated film with 10 minutes of added footage
- Widescreen version of the R-rated film
- Two commentaries by writer-director David Goyer, Jessica Biel, Ryan Reynolds, producer, and crew
- Disc Two
- 16-part behind-the-scenes documentary "Daywalkers, Nightstalkers & Familiars: Inside the World of Blade: Trinity"
- Alternate ending
- Blooper reel
- Goyer on Goyer: The writer interviews the director
- Galleries: VFX progression and weapons
- Theatrical trailer