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In the pilot, after first hearing about vampires but before she finds out that they're actually real, Krista sarcastically asks if werewolves are real too and is told that werewolves are "my colleague Marc Spector's realm of expertise." Spector is the alter ego of the Marvel superhero Moon Knight, who first appeared in the 1970s comic book series Werewolf by Night.
Marcus Van Sciver's last name is an homage to comic book artist Ethan Van Sciver, who worked with series writer and executive producerGeoff Johns.
The series contains numerous references to both the comics and the film series. Recurring villains Steppin' Razor, Thorne, and Damek are based on characters from the comics, footage from the first film is used briefly in the pilot, and Abraham Whistler (Blade's human mentor in the films) is mentioned in the pilot and later appears (in flashback) in the episode Sacrifice.
In the flashback episode where we see Blade as a child, a friend gives him a copy of an Avengers comic book. Series writer Geoff Johns had a stint as a writer for the Avengers.